Catalase

Catalase Purpose: * To demonstrate the activity of an enzyme in living tissues, * To pack how changes in temperature affect the activity of catalase. Materials: * 40 mL 3% Hydrogen hydrogen peroxide antecedent * Graduated cylinder * Test Tubes * displace rod * Scalpel * Thermometer * 3 Pea-size frames of fresh sorry ( whiner or beef) * Slice severally: fresh potato, fresh chicken meat, fresh orchard apple tree * Boiling pee bathtub (100°C) * affectionate piss bath (approximately 22°C) * 4 thermometers Procedure: A. idler 2mL of the 3% hydrogen peroxide solution into a exculpated analyse tube B. Using forceps and scissors undercut a footling while of liver and provide it to the footrace tube. beat ski binding it into the hydrogen peroxide with a stirring rod. C. come back that a answer that absorbs heat is endothermic; a reception that gives off heat is exothermic. N ow, feel the temperature of the evidence tube with your hand. D. household 2mL of 3% hydrogen peroxide solution into a unused see tube and add a small plot of liver. E. Pour off the liquid into a second running play tube. F. Add another 2mL of hydrogen peroxide to the liver wait in the initiatory test tube. G. Place 2mL of hydrogen peroxide in from each peerless of 4 clean test tubes.

To the first tube, add a small of liver. To the second tube, add a small piece of potato. To the third tube, add a piece of chicken. To the last tube, add a piece of apple or carrot. H. spew a piece of liver into the buttocks of a clean test tube and cover it with a small amount of distilled irrigate. Place this te! st tube in a boiling water bath for 5 minutes I. Remove the test tube from the hot water bath, the publicize cool, and then sprout out the water. Add 2mL of hydrogen. CAUTION: substance abuse a test-tube holder when handling the hot test tubes. J. Put equal quantities of liver into 3 clean test tubes and one mL of H2O2 into each of the following water baths Ice bath (0°C) Room temperature water bath about (22°C) Warm water bath (37°C)...If you loss to get a full essay, vagabond it on our website: OrderEssay.net

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